Evaluación in vitro de la actividad inhibidora de fracciones de Passiflora edulis Sims. (Passifloracea) sobre la enzima convertidora de angiotensina
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Lizalda Aponte, Christian Fernando | 2019-07-25
Currently, the prevalence of hypertensive patients is increasing due to psychosocial, behavioral, inherited factors, among others. The use of drugs could lead to the appearance of undesirable effects during the treatment of the disease. Traditional medicine with plants provides alternatives to show potential therapeutic candidates. There is evidence of the effect of Passiflora edulis Sims juice. on the angiotensin-converting enzyme (ACE). In this work, 4 large fractions were obtained by column chromatography using DIAION HP-20 from the juice
of Passiflora edulis Sims., The separation was monitored by U.V. vis analysis and CCD chromatography; the activity of the RCT in blood serum was evaluated, using the Ronca- Testoni method (74), modified by the Biochemistry and Genetics laboratory of the University
of Quindio. We included 5 healthy volunteers without diagnosis of hypertension and with informed consent. Fractions A and B were evaluated at 4 concentrations (10 μg / mL, 20 μg /mL, 50 μg / mL, 100 μg / mL). Both fractions showed inhibitory activity in vitro on RCTs similar
to the drug Captopril (ActA = 40.60 + 22.07, ActB = 44.74 + 24.92, Capt = 41.79 + 32.03, White = 66.33 +23.6 μg / mL). The percentage of ACE inhibition activity for the concentrations was approximately 38.79%. The values of the ACE inhibitory activity due to the effect of the
fraction at different concentrations were not statistically significant. It was evidenced by FTIR spectroscopy the existence of metabolites with characteristics of aromatic carboxylic compounds of unsaturated and aliphatic chains, possibly phenolic esters, as major compounds in both fractions. It is proposed that the inhibition of the enzyme may be
mediated by the interactions of the functional groups with the zinc atom of the metalloproteinase and the formation of hydrogen bonds between the phenolic metabolites and the amino acids of the catalytic site. The metabolites of these fractions could act as adjuvants by having an enzymatic activity close to that of the control medication.
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